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Cell Culture

LC Lili Chen
JZ Jiajia Zhao
QT Qingming Tang
HL Honggui Li
CZ Chenguang Zhang
RY Ran Yu
YZ Yan Zhao
YH Yuqing Huo
CW Chaodong Wu
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Human tongue carcinoma SCC9 cells and control KC cells were commercially obtained from ATCC. SCC9 and KC cells were maintained in Dulbecco’s Modified Eagle’s/Ham’s F12 Medium (Sigma, MO, USA) supplemented with 10% heated-inactivated fetal bovine serum, 0.5% penicillin, 0.5% streptomycin, 1.2 g/L sodium bicarbonate, 400 μg/L hydrocortisone in a humidified 5% CO2 atmosphere at 37 °C. Culture medium was changed every 3 days.

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