Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Murine model of sepsis.

LH Luke D. Handke
AG Alexey V. Gribenko
YT Yekaterina Timofeyeva
IS Ingrid L. Scully
AA Annaliesa S. Anderson
request Request a Protocol
ask Ask a question
Favorite

Female 9-to-12-week-old CD1 mice (Charles River Laboratories, Inc., Wilmington, MA) were used for virulence studies. S. aureus challenge strains were cultured in TSB medium, and 9 or 10 mice per bacterial strain were inoculated with approximately 1 × 108 CFU via tail vein injection. Survival was monitored for at least 4 days postchallenge. Data were analyzed using GraphPad Prism 6 software (GraphPad Software, Inc., La Jolla, CA). Kaplan-Meier survival curves were plotted, and statistical significance was assessed with log rank (Mantel-Cox) tests. All animal work was performed in strict accordance with approved Institutional Animal Care and Use Committee (IACUC) protocols at an American Association of Laboratory Animal Science (AALAS)-accredited facility (Pfizer, Inc., Pearl River, NY).

Supplemental Materials and Methods. Download TEXT S1, DOCX file, 0.02 MB.

Copyright and License information:  ©2018 Handke et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A