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Cell culture

SO Shoko Ohno
HY Hideki Yokoi
KM Kiyoshi Mori
MK Masato Kasahara
KK Koichiro Kuwahara
JF Junji Fujikura
MN Masaki Naito
TK Takashige Kuwabara
HI Hirotaka Imamaki
AI Akira Ishii
MS Moin A. Saleem
TN Tomohiro Numata
YM Yasuo Mori
KN Kazuwa Nakao
MY Motoko Yanagita
MM Masashi Mukoyama
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Conditionally immortalized human podocytes were cultured as described previously36,37. Differentiated podocytes were serum-starved for 24 h and then pretreated with 100 nM ω-conotoxin (Peptide Institute, Osaka, Japan)38, 10 μM cilnidipine or 10 μM nitrendipine at 1 h before the stimulation of recombinant human TGF-β1 (R&D Systems, Minneapolis, MN). Cells were harvested after 24 h, and the expression level of nephrin was assayed by real-time RT-PCR analysis (n = 5–12).

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