Water soluble tetrazolium salt (WST-1) cell proliferation and viability assay

HL Hyun Jik Lee
YJ Young Hyun Jung
JO Ji Young Oh
GC Gee Euhn Choi
CC Chang Woo Chae
JK Jun Sung Kim
JL Jae Ryong Lim
SK Seo Yihl Kim
SL Sei-Jung Lee
JS Je Kyung Seong
HH Ho Jae Han
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The proliferation and viability of the UCB-MSCs were determined with the WST-1 cell viability assay kit (EZ-Cytox™; Daeil Labservice, Seoul, Korea, #EZ-1000). The assay was performed according to the manufacturer’s instructions. Briefly, BICD1, BICD2 or NT siRNA-transfected UCB-MSCs cultured in 96-well plates were treated with normoxia or hypoxia for 24 h. Cells were incubated in 10 μL of EZ-Cytox™ solution in 100 μL of medium for 30 min in a cell incubator at 37 ℃. Then, the absorbance was measured with a microplate spectrophotometer (Epoch 2™; BioTek, Winooski, VT, USA) at 450 nm.

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