Blood samples and biochemistry assessment

All blood samples were taken from the antecubital vein while the participant was in a sitting position. Blood samples were taken before the run – sample »A«, 1 hour after the start – sample »B«, at the end of the run (2 hours after the start) – sample »C«, 1 hour after the end of the run (3 hours after the start) – sample »D« and 24 hours after the end of the run – sample »E« (Figure 1). In sample »A«, three blood samples were taken – 1x VACUETTE® TUBE 4 ml Kalium Ethylene-Diamine-Tetra-Acetic acid (K3EDTA) and 2x VACUETTE® TUBE 4 mL Lithium heparine (LiHep) Separator, Greiner Bio-One GmbH, Kremsmünster, Austria. At all other time points, 2 blood LiHep samples were taken. Urine samples were taken at the same time as samples »A«, »D« and »E« (1x VACUETTE® Urine tube). All LiHep samples were centrifuged 15 minutes after collection at 2000 g for 10 minutes. Samples were stored at −70 °C prior to analysis.