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Mycobacteria bovis (MOI: 10:1) and HBD3 protein were separately co-incubated with A549 and RAW 264.7 cells for 24 h. Cell apoptosis was then evaluated by DeadEnd TM Fluorometric Tunel System (Promega Corporation, Madison, WI, USA) and cell nucleus dying by PI according to the instruction book (Sigma Co, St. Louis, MO). Cell apoptosis ratio was then detected by flow cytometry.
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