Eight to nine week old male C57BL/6 mice and nine to ten week old male ICR mice were obtained from Envigo (Indianapolis, IN). Nine to thirteen weeks old GPR40 and GPR120 knock-out (KO) mice were generated by Taconic (Hudson, NY). All animals were singly housed in a temperature-controlled (24 °C) facility using a 12-h light/dark cycle (light on 0600) and had ad libitum access to food and water unless otherwise stated. All in vivo experiments were performed in compliance with the policies of the Animal Care and Use Committee of Eli Lilly and Company, in conjunction with the American Association for the Accreditation of Laboratory Animal Care-approved guidelines.
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