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Cytokine analysis with LUMINEX and LEGENDplex

SR Sandra Rosskopf
BJ Beatrice Jahn-Schmid
KS Klaus Georg Schmetterer
GZ Gerhard J. Zlabinger
PS Peter Steinberger
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Supernatants of the TCC proliferation assays were harvested at day 5–6 and pooled from duplicate wells. IL-10, IFN-γ, TNF-α, IL-4 and IL-13 were measured with the Luminex 100 system (Luminex Inc., Austin, TX) according to the manufacturer’s instructions. Supernatants of the PBMC proliferation assays were harvested at day 6–7, pooled from triplicate wells and stored at −20 °C. IL-5, IL-10, IL-13, IL-17F, IFN-γ and TNF-α were determined using the LEGENDplex human TH 1/2 cytokine panel (13-plex, Biolegend) according to the manufacturer’s instructions. Cytokine content of each donor is represented by a single dot.

Copyright and License information: The Author(s) ©2018
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