In vitro drug releases of DOX and SF from PEG-CD/AD/SF nanoparticles

The in vitro releases of DOX and SF from PEG-CD/AD/SF nanoparticles were evaluated using dynamic dialysis method in four kinds of release media (I: pH 7.4 PBS; II: pH 7.4 PBS with 10 mM DTT; III: pH 5.0 PBS; IV: pH 5.0 PBS with 10 mM DTT). Briefly, PEG-CD/AD/SF nanoparticle solutions (1 mL) were placed into dialysis bags (MWCO 7000) and dialyzed against above media at 37°C in the air bath shaking with 100 rpm. At designated time points, 0.5 mL of release media was withdrawn and replaced with an equal volume of fresh release media. The amounts of DOX and SF in the release media were determined by UV/Vis spectrophotometer (Beckman DU-640, USA) at a wavelength of 490 and 267 nm, respectively (Malarvizhi et al., 2014). The release tests were repeated for three times and the accumulative releases of drug were calculated.