Cell culture, siRNA knockdown, and plasmid transfection

YY Yanan Yue
JL Jun Liu
XC Xiaolong Cui
JC Jie Cao
GL Guanzheng Luo
ZZ Zezhou Zhang
TC Tao Cheng
MG Minsong Gao
XS Xiao Shu
HM Honghui Ma
FW Fengqin Wang
XW Xinxia Wang
BS Bin Shen
YW Yizhen Wang
XF Xinhua Feng
CH Chuan He
JL Jianzhao Liu
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Human HeLa cell line was grown in Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose media (HyCloneSH30243.01) supplemented with 10% fetal bovine serum (FBS; Gibco 10270) and 1% 100× Pen Strep (HyClone SV30010). HEK 293T was grown in DMEM/high-glucose media (HyCloneSH30027.01) and was only used in the protein expression test. All the siRNAs were ordered from Qiagen with sequences shown in Table S9. All the antibodies used in this work were listed in Table S10. Transfection was achieved by using Lipofectamine RNAiMAX (Invitrogen) for siRNA, or Lipofectamine 2000 (Invitrogen) for the plasmid following the manufacturer’s protocols.

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