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Total of 6 samples, including J1-MT-MEF-P30, J1-MT-3T3-P20, J1-MMC-MEF and miPS-MT-MEF-P29, miPS-MT-3T3-P20, miPS-MMC-MEF, were harvested and injected subcutaneously into dorsal flanks of immunodeficient mice (5 × 106 cells per injection site). Control mice received 1xPBS only. After 4 weeks, the nude mice were sacrificed and the teratomas were collected, fixed with 4% paraformaldehyde, and tissues were stained with Hematoxylin and Eosin as the previous description47,48. Images were obtained under a Nikon microscope.
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