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The LDH-Cytotoxicity Colorimetric Assay Kit II (#K313-500, BioVision, USA) was used to detect cell injury. The experimental steps were strictly performed according to the manufacturer’s manual. Briefly, medium solution (10 μl per well) was added to each neuronal culture well of a 24-well plate in an optically clear 96-well plate. Then, 100 μl of LDH Reaction Mix was added to each well, mixed, and incubated for 30 min at room temperature. The absorbance at 450 nm was measured with a microplate reader (Infinite M200, TECAN).
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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