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Total RNA was extracted using TRIzol Reagent (Life Technologies) following the manufacturer's instructions. RNA was dissolved in RNase-free water fortified with RNase inhibitors (RNaseSUPERase-In; Life Technologies). The amount and purity (A260/A280 ratio) of the extracted RNA were assessed spectrophotometrically. cDNA was synthesized by reverse transcription from 2 μg RNA with a commercial kit and random primers (High-Capacity cDNA Reverse Transcription Kit; Life Technologies) following the manufacturer's instructions.
Copyright and License information: ©2018 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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