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Aβ cortical injection

JL Ju Youn Lee
SH Seung Hoon Han
MP Min Hee Park
BB Bosung Baek
IS Im-Sook Song
MC Min-Koo Choi
YT Yoh Takuwa
HR Hoon Ryu
SK Seung Hyun Kim
XH Xingxuan He
ES Edward H. Schuchman
JB Jae-Sung Bae
HJ Hee Kyung Jin
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CamK2-cre;SphK1flox/flox mice (2-month-old) were injected with Aβ (10 μM) at cortex of brain. After anesthesia with a combination of 100 mg/kg ketamine and 10 mg/kg xylazine, a stainless steel cannula was implanted in the animal’s cortex using a stereotaxic frame (David Kopf Instrument, Tujunga, CA). The guide cannula was fixed in the cortex brain region according to the following coordinates: 1.8 mm posterior to the bregma, 3 mm bilateral to the midline, and 1.2 mm ventral to the skull surface. The guide cannula allows a stable aperture in the brain by indicating where the treatments can be administrated. The injection of aggregated Aβ was made bilaterally into the cortex using an injection cannula connected to a microsyringe (Hamilton). The volume of injection was 1 μl of 10 μM aggregated Aβ or 1 μl of PBS.

Copyright and License information: The Author(s) ©2018
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