Bioinformatics Analysis

CC Chunhong Cheng
CL Changman Li
DW Diandong Wang
LZ Lifeng Zhai
ZC Zhaoming Cai
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The protein sequences were alimented by ClustalX 1.83 (Thompson et al., 1997), and a phylogenetic tree was constructed using neighbor joining method with the bootstrip values of 1000 by MEGA5 (Tamura et al., 2011). The gene structure analysis was done by the online software1. Promoter analysis of GmNARK was performed using online analysis software of PLACE2 and PlantCARE3. The region located 2 kb upstream of GmNARK coding sequences was used as promoter sequence for analyzing cis-elements. GmNARK protein domain analysis was done by the online software of SMART4 and ExPASy5. The amino acid alignment was done by the online software of WebLogo 3.6

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