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Primary human renal mesangial cells were purchased from ScienCell (Carlsbad, CA), grown in Mesangial Cell Medium (ScienCell) supplemented with 2% FBS and 1% mesangial cell growth supplement (ScienCell). Cells were passaged by trypsinization when confluent and used between the third and ninth passages. Before treatment, cells were cultured in serum-free RPMI medium with 5 mM glucose for 24 h.
Copyright and License information: ©2018 The Author(s).
This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
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