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Quantification of TNF-α and IL-1β by ELISA
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Lung tissues were weighed, homogenized, and subjected to centrifugation maintaining at 4°C. Tissue supernatants were stored at -80°C in aliquots or used immediately to estimate cytokine expression. Pro-inflammatory cytokines including TNF-α and IL-1β were evaluated for their expression in lung tissues using commercially available ELISA kits (eBioscience, TNF-α Catalogue No-50-173-67; IL-1β Catalogue No-50-112-8807).
Copyright and License information: ©2018 Bale, Venkatesh, Sunkoju and Godugu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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