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After 21 days, cultures were rinsed with 70% (v/v) isopropanol for 5 min and differentiation was assessed qualitatively by specific staining of lipid droplets with 0.5% Oil Red O solution (Sigma-Aldrich, Poole, UK). Intracellular lipid accumulation was quantified using the AdipoRed™ Adipogenesis Assay (Lonza) following the manufacturer’s protocol. Briefly, cells were pre-washed with PBS and incubated with AdipoRed™ Reagent for 10 min. Fluorescence was measured using a plate reader (λexc/λem 485/572 nm).
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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