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Cell viability assay

KB Katerina Brustikova
DS David Sedlak
JK Jana Kubikova
CS Ctibor Skuta
KS Katerina Solcova
RM Radek Malik
PB Petr Bartunek
PS Petr Svoboda
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Cell viability was assessed by determining the level of intracellular ATP using the ATPlite™ (Perkin Elmer) luminescent assay. The assay was carried out with 3T3 cells, in parallel with a corresponding reporter assay and under exactly the same conditions as the reporter assay. At the end of the incubation with test compounds, 2.5 μl aliquots of the reagent were dispensed to 1536-well plates using Multidrop Combi. The assay plates were shaken vigorously for 3 min. After another 10 min, luminescence was recorded in the multimode plate reader Envision (Perkin Elmer). Data were collected and processed in the LIMS system ScreenX.

Copyright and License information:  ©2018 Brustikova, Sedlak, Kubikova, Skuta, Solcova, Malik, Bartunek and Svoboda.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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