Thymi were fixed with modified Davidson’s fluid and dehydrated with Li-EtOH and EtOH in a stepwise fashion and finally embedded in paraffin; RNA in situ hybridization (ISH) on paraffin sections was performed using DIG-labelled probes32. The following probes were used: Ccl25 (nt 119-553 in Genbank accession number NM_009138); Cxcl12 (nt 64-479 in Genbank accession number NM_ 021704); Foxn1 (nt 2181-3584 in Genbank accession number XM_006532266.3); Dll4 (nt 2271-3421 in Genbank accession number BC049130); Prss16 (nt: 297-700 in Genbank accession number NM_019429.2); Psmb11 (nt 394-862 in Genbank accession number AB299436.1).
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