4.3. Protein Expression Analysis

Cell lysates of SRA-hLECs and mLECs were prepared in an ice-cold radioimmune precipitation buffer, and protein blot analysis was performed as described previously [78,79,80]. The membranes were probed with anti-HA (ab 18181 and ab9110, Abcam^®, Cambridge, MA, USA), Anti-Sp1, Anti-Prdx6 antibody (LF-PA0011 and LF-MA0018, Ab Frontier, South Korea), or β-actin (A2066, Sigma-Aldrich, St. Loius, MO, USA) as internal control to monitor those protein expressions. After secondary antibody (sc-2354 and sc-2768, Santa Cruz Biotechnology, Dallas, TX, USA), protein bands were visualized by incubating the membrane with luminol reagent (sc-2048; Santa Cruz, Santa Cruz Biotechnology, Dallas, TX, USA) and images were recorded with a FUJIFILM-LAS-4000 luminescent image analyzer (FUJIFILM Medical Systems Inc., Hanover Park, IL, USA).