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Mouse MASTL cDNA was amplified by using PCR and cloned into the pcDNA3.1 vector. This construct was transfected by using TransIT 2020 (Mirus Bio, Madison, WI) to induce the ectopic overexpression of mouse MASTL. MCF7 cells (7.5 × 105) were seeded in a 60-mm dish with siRNA assembled by G-fectin. After the cells were left to adhere to the dish for 6 h, 2 μg of mMASTL-pcDNA3.1 or empty-pcDNA3.1 vector applied to TransIT 2020 was added to the cells.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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