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Intact SMGs were surgically removed from each mouse following euthanasia. SMGs were either lysed for RNA or protein, cryopreserved, or fixed in paraformaldehyde (PFA) 4%. After PFA fixation, 4-μm paraffin sections were stained with H&E and visualized in an Axiovert 25 light microscope (Zeiss, NY, USA) at 40× magnification. H&E images were taken with a Nikon 5000 camera (Nikon, Japan). Cryopreserved in vivo SMG tissues were used for immunofluorescence analysis.
Copyright and License information: ©2018
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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