Total RNA Extraction

JC Jing Chen
MG Marina Gálvez-Peralta
XZ Xiang Zhang
JD Jingyuan Deng
ZL Zijuan Liu
DN Daniel W. Nebert
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RNeasy Micro kit (Qiagen) was used for total RNA extraction. Each sample, together with Buffer RLT, were added to ceramic-beads (1.4-mm) in tubes and homogenized by using the Precellys homogenizer (Cayman Chemical Company; Ann Arbor, MI). RNA extraction was then performed, following manufacturer’s recommendations.

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