Total RNA was prepared from BMDMs isolated from 8-week-old male Crif1f/f mice. RNA amplification and labeling were performed using Cyanine 3-labeled cRNA (complementary RNA) was generated from Agilent’s Low RNA Input Linear Amplification kit with 500 ng total RNA (Agilent Technologies, Santa Clara, CA, USA). Labeled cRNA was applied microarray (Agilent technologies, 8 × 60 K) using Agilent’s Gene Expression Hybridization Kit (Agilent Technologies). Data were extracted on a G2565CA DNA Microarray Scanner using the Agilent Feature Extraction Software (Agilent Technologies) with default settings.
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