Microarray analysis of cDNA

SJ Saet-Byel Jung
MC Min Jeong Choi
DR Dongryeol Ryu
HY Hyon-Seung Yi
SL Seong Eun Lee
JC Joon Young Chang
HC Hyo Kyun Chung
YK Yong Kyung Kim
SK Seul Gi Kang
JL Ju Hee Lee
KK Koon Soon Kim
HK Hyun Jin Kim
CK Cuk-Seong Kim
CL Chul-Ho Lee
RW Robert W. Williams
HK Hail Kim
HL Heung Kyu Lee
JA Johan Auwerx
MS Minho Shong
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Total RNA was prepared from BMDMs isolated from 8-week-old male Crif1f/f mice. RNA amplification and labeling were performed using Cyanine 3-labeled cRNA (complementary RNA) was generated from Agilent’s Low RNA Input Linear Amplification kit with 500 ng total RNA (Agilent Technologies, Santa Clara, CA, USA). Labeled cRNA was applied microarray (Agilent technologies, 8 × 60 K) using Agilent’s Gene Expression Hybridization Kit (Agilent Technologies). Data were extracted on a G2565CA DNA Microarray Scanner using the Agilent Feature Extraction Software (Agilent Technologies) with default settings.

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