For experiments using rIL-4, C57BL/6 mice were fed a HFD and given intraperitoneal injections of rIL-4 (2 μg/mouse, 214-14, PeproTech, Rocky Hill, NJ, USA) or PBS alone every day for 2 weeks. For experiments using rGDF15, MacWT, MacHO, ob/+ and ob/ob mice were given intraperitoneal injections of rGDF15 (8 μg/mouse, 957-GD/CF, R&D Systems) or 4 mM HCl alone every other day for 2 weeks.
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