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Cells were treated with 25 μM Cd for the indicated times, then washed with phosphate-buffered saline (PBS), harvested using trypsin, suspended in PBS, and incubated with 4 μM fluo-4 at 37 °C for 1 h. The cells were centrifuged at 1000×g for 5 min, washed three times with PBS, resuspended in PBS, and incubated at 37 °C for 20 min. Intracellular Ca2+ was analyzed using flow cytometry (FACSCanto; BD Biosciences).
Copyright and License information: The Author(s) ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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