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To determine the phenol tolerance, 100 µl of overnight grown culture of each isolate was inoculated into 900 µl MRS broth supplemented with different phenol concentration (0.1–0.5%) and incubated at 37 °C for 24 h. Viable cells were counted by plating serial dilutions on MRS agar and incubation anaerobically at 37 °C for 48 h. Phenol tolerance cells were assessed by calculating the ratio of survived viable cell cultured (%) on MRS agar compared to the control (without phenol).
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