Histology

YP Yuliya Pepelyayeva
DR David P. W. Rastall
YA Yasser A. Aldhamen
PO Patrick O’Connell
SR Sandra Raehtz
FA Fadel S. Alyaqoub
MB Maja K. Blake
AR Ashley M. Raedy
AA Ariana M. Angarita
AA Abdulraouf M. Abbas
CP Cristiane N. Pereira-Hicks
SR Sarah G. Roosa
LM Laura McCabe
AA Andrea Amalfitano
request Request a Protocol
ask Ask a question
Favorite

Colon sections were harvested from DSS-treated WT or ERAP1−/− mice at day 7, fixed, stained with hematoxylin and eosin (H&E) and scored, as previously described22. Mice received a score of 0–3 for inflammation with 1, 2, and 3 corresponding to lymphocytic infiltration of the epithelium, mucosa, and transmural inflammation, respectively. Colons were then scored 0–3 for pathologic changes to intestinal architecture with 1, 2, and 3 corresponding to focal erosions, extensive erosions, and extensive ulcerations with granulation tissue, respectively.

Spines were harvested, fixed for 48–72, hours and further processed for H&E and immunohistochemistry as described below.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A