Colon sections were harvested from DSS-treated WT or ERAP1−/− mice at day 7, fixed, stained with hematoxylin and eosin (H&E) and scored, as previously described22. Mice received a score of 0–3 for inflammation with 1, 2, and 3 corresponding to lymphocytic infiltration of the epithelium, mucosa, and transmural inflammation, respectively. Colons were then scored 0–3 for pathologic changes to intestinal architecture with 1, 2, and 3 corresponding to focal erosions, extensive erosions, and extensive ulcerations with granulation tissue, respectively.
Spines were harvested, fixed for 48–72, hours and further processed for H&E and immunohistochemistry as described below.
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