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Senescence-associated β-galactosidase staining
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To examine senescence-associated β-galactosidase (SA β-gal activity), cultured cells were stained using a senescence cell histochemical staining kit (Merck KGaA) according to the manufacturer instructions. Briefly, overconfluent cultures on day 14 were subcultured in 60-mm culture dishes. The cell aggregates after SA β-gal staining were examined using a Biorevo BZ-9000 microscope (Keyence).
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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