Intraluminal SCFA (acetate, butyrate, and propionate) were extracted from 20 randomly selected samples using isopropyl alcohol from 5-month old infants’ fecal contents as described previously69. The amount of acetic, propionic, and butyric acid were analyzed from fecal samples via direct-injection gas chromatography as previously described70, and expressed as mass % (g of SCFA per g of dry weight stool).
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