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Analysis of PI3K/Akt and MAPK/ERK pathway activation
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RCC cells (786-O and ACHN) were seeded at a density of 5000 cells per well in 6-well plates and were treated with increasing concentrations of WIN-55 as described above. The Muse™ PI3K/MAPK Dual Pathway Activation Kit (MCH200108) was used to evaluate the activation of the PI3K/Akt and MAPK/ERK signaling pathways simultaneously by WIN-55 treatment. The assay was performed according to the manufacturer user’s guide.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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