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The model saliva was prepared in 0.1 M phosphate buffered saline (PBS) according to Green26, using bovine submaxillary mucin (Type I-S) and α-amylase from porcine pancreas (Type VI-B), purchased from Sigma Aldrich (Dorset, UK). The final protein concentrations in the model saliva were adjusted to the concentrations of mucin and α-amylase in human saliva, neglecting the presence of SIgA and other proteins.
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