2.7. Statistical analysis

GraphPad Prism 7.0 a software (GraphPad Software, La Jolla, CA, USA) was used for all statistical analyses. All in vitro experiments were performed in triplicate, and the mean for each individual animal was used for statistical analysis. The quantitative data were tested for normality using the Shapiro‐Wilk test. Non‐normally distributed continuous variables were compared using Mann‐Whitney U test. Student t test was used for evaluating of normally distributed variables. P < 0.05 was considered as statistically significant.