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The collagen contents of samples were quantified using a Sircol collagen assay kit (Biocolor, Carrickfergus, UK) according to the manufacturer’s instructions. This step was done following collagen extraction by incubation for overnight in a pepsin-containing solution at 65 °C. The absorbance of each sample was determined at 550 nm using a microplate reader (Molecular Devices, CA, USA) and the collagen quantity was calculated by usage of a standard curve generated with water-soluble denatured collagen from bovine skin.
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