MRI Protocol

Magnetic resonance images were obtained on a Siemens MAGNETOM TIM Trio 3 Tesla system (Erlangen, Germany). We acquired the following scans: three-dimensional T1-weighted high resolution anatomical, T2-weighted turbo spin echo, fluid-attenuated inversion recovery (FLAIR), and resting state functional magnetic resonance imaging (rs-fMRI) scans. Parameters for each scan are listed here. T1-weighted: TR = 2000 ms; TE = 2.63 ms; flip angle = 9°; voxel size = 1 × 1 × 1 mm³; field of view (FOV) = 192 × 256 mm²; matrix size = 192 × 256 voxels; 160 axial slices; slice thickness = 1 mm. T2-weighted: TR = 2900 ms; TE 1 = 19 ms; TE 2 = 102 ms; flip angle = 180°; voxel size = 0.9 × 0.9 × 3 mm³; FOV = 185.68 × 220 mm²; matrix size = 216 × 256 voxels; 48 axial slices; slice thickness = 3 mm. FLAIR: TR = 9000 ms; TE = 96 ms; flip angle = 165°; voxel size = 0.9 × 0.9 × 5 mm³; FOV = 185.5 × 224 mm²; matrix size = 212 × 256 voxels; 32 axial slices; slice thickness = 5 mm. Rs-fMRI: TR = 2000 ms; TE = 27 ms; flip angle = 70°; voxel size = 3 × 3 × 3 mm³; 180 volumes; FOV = 192 × 192 mm²; matrix size = 64 × 64 voxels; 40 axial slices; slice thickness = 3 mm; gap = 0.5 mm. Participants fixated on a cross during the rs-fMRI scan. We recorded physiologic information during the rs-fMRI scan using a pulse oximeter on the finger to measure heart beat and a respiration belt tied around the participant’s chest to measure respiration.