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Microarray analysis

MW Michael M. Wang
XZ Xiaojie Zhang
SL Soo Jung Lee
SM Snehaa Maripudi
RK Richard F. Keep
AJ Allison M. Johnson
SS Svetlana M. Stamatovic
AA Anuska V. Andjelkovic
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Transcriptome analysis was performed using Affymetrix Human Gene ST 2.1 strips that were processed in the University of Michigan Microarray Facility. Labeled cDNA was prepared using a WT Pico Kit (Affymetrix). Probesets that yielded differences of more that 2-fold between PRX and control groups were considered potentially differentially expressed. Two independent replicates were performed, and the 66 genes that overlapped between two experiments were presented in the final results. A subset of these genes were assessed by quantitative RT-PCR to validate in independent cell groups, performed in triplicate, whether expression was altered by PRX expression.

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