2.4. Acetic acid induced writhing’s test

The analgesic evaluation of methanolic extracts of MB and BA were estimated by acetic acid induced writhing’s test (Koster, 1959). Albino mice of each sex were randomly divided into four groups comprising of six in each. Normal saline at concentration of 10 mL/Kg was injected to Group I as control, diclofenac sodium as a standard drug was injected to Group II at concentration of 10 mg/kg, while different concentrations of methanolic extracts (50, 100 and 150 mg/kg), through i,p route were injected respectively. Acetic acid (1%) was injected after 30 min after the above treatment. After five minutes the abdominal writhes were counted for the periods of ten minutes.