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Hypoxia response element (HRE) promoter activity with dual luciferase assay

QL Qingguo Li
YL Yaqi Li
LL Lei Liang
JL Jing Li
DL Dakui Luo
QL Qi Liu
SC Sanjun Cai
XL Xinxiang Li
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HEK-293 T Cells were seeded into 96-well culture plates and transfected by using Lipofectamine™ 2000 (Invitrogen). 200 ng of pCDH-CMV-MCS-EF1-KL-Puro expressing vector, HRE-luciferase plasmid (Addgene, 26,731) and the Renilla luciferase expression vector, pRL-TK (Promega), were transfected into cells [35]. Forty-eight hours after transfection, cells were assayed for both firefly and renilla luciferase activities using a dual-luciferase system (Promega), as described according to the manufacturer’s protocol. All experiments were performed in triplicate. Data is represented as mean ± SD.

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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