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PI3K kinase assay
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ADP-Glo luminescent assay for PI3Kγ was performed according to the standard protocols of Promega. In brief, the kinase reaction was done in 96-well plate. Each well was loaded with test items and reaction buffer containing PI substrate and the PI3K proteins were then added. The reaction was started by the addition of PIP2 and ATP prepared in the reaction buffer and ran for 60 min and subsequently terminated by the addition of ADP-Glo reagent, then read the plate for luminescence detection.
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