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Genotyping of the BAC Tg mice was performed by PCR analysis. PCR assays were designed using primers targeting the T7 and SP6 sequences in BAC vector pBACe3.6, and primers 59P20T7qR and 59P20SP6qR, which were specific to the 5′ and 3′ boundaries of mouse genomic DNA within the BAC clone. All primers used for PCR, qPCR and ddPCR analysis are listed in Supplementary Table 1.
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