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Chromatin immunoprecipitation (ChIP) assay
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ChIP assays were performed by using the Magna ChIP Kit (Millipore, Bedford, MA) in accordance with the manufacturer’s directions. HuCCT1 and RBE cells were treated with formaldehyde to generate DNA-protein cross-links. Cell lysates were sonicated to generate chromatin fragments of 200–300 bp, and the lysates were immunoprecipitated with specific antibodies or IgG as the control. The precipitated chromatin DNA was recovered and measured by qPCR.
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