Statistical Analysis

The data was presented as mean (±SEM) and checked for normality using D’Agostino and Pearson omnibus normality test. Nonparametric data was compared using Mann Whitney test, while normal data was compared using Student’s t test. The mRNA levels of TLRs in vitreous humor were compared using Mann Whitney test. The correlation between fold changes of various TLRs and between TLR expression, CD4+ Teff cell responses and ocular inflammation scores was tested using Spearman correlation test. The effect of TLR stimulation on proliferation of Teff cells within non-uveitis control group was compared using Wilcoxon matched-pairs signed rank test while that in IOTB using paired t test. The effect of TLR stimulation on Treg induction, intracellular cytokine expression and mRNA levels of TLR9 signalling pathway genes, between the subject groups were compared using unpaired t test or Mann Whitney test, depending on data distribution. All statistical analysis was performed using Graphpad prism software version 5.03.