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To detect apoptosis, TUNEL assay were performed with in situ Cell Death Detection kit (TUNEL, Roche) according to the manufacturer's instructions. The number of TUNEL-positive cardiomyocytes was counted in 10 randomly selected fields for each animal. The percentage of apoptotic cells was determined by the ratio of apoptotic cells to total cells.
Copyright and License information: ©2018 Qi, Wang, Yang, Wang, Xu, Chen, Yuan, Lu and Duan.
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