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Microbial culture

MH Marion Herisse
JP Jessica L. Porter
RG Romain Guerillot
TT Takehiro Tomita
AS Anders Goncalves Da Silva
TS Torsten Seemann
BH Benjamin P. Howden
TS Timothy P. Stinear
SP Sacha J. Pidot
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Nocardia strains were routinely cultured in Brain Heart Infusion (BHI) broth or on BHI agar plates (Difco). E. coli strains were cultured in Luria broth or on Luria agar plates. Streptomyces species were cultured on MS agar for conjugation and isolation of transconjugants (Kieser et al., 2000). All strains used in this study are listed in Table S1. The plasmid pRT801, an E. coli-Streptomyces integrating vector containing the BT1 int gene and attP site, aac3(iv) (encoding apramycin resistance) and a Streptomyces oriT for conjugative transfer, was used in this study and has been previously published (Gregory, Till & Smith, 2003).

Copyright and License information:  ©2018 Herisse et al.
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