RNA extraction and cDNA synthesis

ZM Zaid H. Maayah
JL Jody Levasseur
RP Ramanaguru Siva Piragasam
GA Ghada Abdelhamid
JD Jason R. B. Dyck
RF Richard P. Fahlman
AS Arno G. Siraki
AE Ayman O. S. El-Kadi
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TRIzol reagent (Invitrogen®) and the High-Capacity cDNA reverse transcription kit (Applied Biosystems) were used to isolate total RNA from frozen tissues or treated cells and synthesize cDNA, respectively, according to the manufacturer’s instructions as described previously14,15.

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