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Potassium hexachloroplatinate (K2PtCl6), graphite powder (44 μm size), ascorbic acid (AA), uric acid (UA), β-D(+) glucose, and hydrogen peroxide (30%), chitosan (white mushroom, higher molecular weight), nafion (5%), activated carbon, N-hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N- ethylcarbodiimidehydrochloride (EDC) were purchased from Aldrich Co. (St. Louis, USA). The stock β-D(+) glucose (99.5%, Sigma) solution was prepared by diluting it in 0.05 M PBS (pH 7.4) solution, and all other solutions were prepared with deionized water (resistivity ≥18 MΩ-cm).

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