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Synchronised L1 hl-2(ok818) animals were plated onto gfp or control (Arabidopsis thaliana gene Lhcb4.3 (pCB19), which has no homology to any C. elegans gene) RNAi bacteria. Animals were scored 3 days later for the presence of GFP (oocytes were visualised by fluorescence microscopy using a 63x objective), with silenced animals bleached treated to collect progeny which were transferred OP50 plates for subsequent generations.
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