Evaluation of LAMP using S. scitamineum inoculated samples

Two-bud sets of ROC22 were inoculated with 0.5 μL of the S. scitamineum suspension containing 5 × 10⁶ spores/mL in 0.01% (v/v) Tween-20. All the treated samples were planted in sterile soil and cultivated at 28 °C in 16 h of light and 8 h of darkness. At each time point of 0 d, 1 d, 2 d, 5 d, 7 d, 10 d and 14 d, five inoculated buds were collected for DNA extraction using the CTAB method. These DNA samples (1 μg) were subjected to the optimized LAMP reaction and conventional PCR (bE gene and Pep1 gene detection) reaction systems. The 2.0% agarose gel electrophoresis was used to detect the amplified product of the conventional PCR.