Nissl staining

TN Thuy-Vi V. Nguyen
MH Megan Hayes
JZ Jacob C. Zbesko
JF Jennifer B. Frye
NC Nicole R. Congrove
NB Nadia P. Belichenko
BM Brian S. McKay
FL Frank M. Longo
KD Kristian P. Doyle
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Mounted, dehydrated brain sections were cleared in xylenes, rehydrated, immersed in 0.5% cresyl violet acetate (Millipore Sigma) solution for 1 minute, rinsed with distilled water, differentiated in 0.25% acetic alcohol, dehydrated though a graded ethanol series, cleared, and coverslipped with mounting media.

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